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Merck KGaA primary mouse monoclonal antibody nucleolin
Analysis of Isolated Fractions (A) Western blot of Golgi (GM130 and MYLK4), nucleus <t>(nucleolin</t> and MYLK4), cytoplasmic (MYLK4), and mitochondrial (NDUFB9) markers in the three fractions collected after the sucrose gradient ultracentrifuge. F1, fraction 1 (top); F2, fraction 2 (half); F3, fraction 3 (bottom). (B) Visualization of isolated Golgi vesicles using transmission electron microscopy (white arrows). Numerous smooth vesicles are seen, mostly formed by a single membrane, apparently empty with little dense content, mainly a light network of granular material. The presence of mitochondrial remains is also observed (black arrow). Bar represents 500 nm.
Primary Mouse Monoclonal Antibody Nucleolin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibody+nucleolin/pmc07580119-12-0-6?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
primary mouse monoclonal antibody nucleolin - by Bioz Stars, 2026-07
90/100 stars

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1) Product Images from "Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient"

Article Title: Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient

Journal: STAR Protocols

doi: 10.1016/j.xpro.2020.100100

Analysis of Isolated Fractions (A) Western blot of Golgi (GM130 and MYLK4), nucleus (nucleolin and MYLK4), cytoplasmic (MYLK4), and mitochondrial (NDUFB9) markers in the three fractions collected after the sucrose gradient ultracentrifuge. F1, fraction 1 (top); F2, fraction 2 (half); F3, fraction 3 (bottom). (B) Visualization of isolated Golgi vesicles using transmission electron microscopy (white arrows). Numerous smooth vesicles are seen, mostly formed by a single membrane, apparently empty with little dense content, mainly a light network of granular material. The presence of mitochondrial remains is also observed (black arrow). Bar represents 500 nm.
Figure Legend Snippet: Analysis of Isolated Fractions (A) Western blot of Golgi (GM130 and MYLK4), nucleus (nucleolin and MYLK4), cytoplasmic (MYLK4), and mitochondrial (NDUFB9) markers in the three fractions collected after the sucrose gradient ultracentrifuge. F1, fraction 1 (top); F2, fraction 2 (half); F3, fraction 3 (bottom). (B) Visualization of isolated Golgi vesicles using transmission electron microscopy (white arrows). Numerous smooth vesicles are seen, mostly formed by a single membrane, apparently empty with little dense content, mainly a light network of granular material. The presence of mitochondrial remains is also observed (black arrow). Bar represents 500 nm.

Techniques Used: Isolation, Western Blot, Transmission Assay, Electron Microscopy, Membrane



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Merck KGaA primary mouse monoclonal antibody nucleolin
Analysis of Isolated Fractions (A) Western blot of Golgi (GM130 and MYLK4), nucleus <t>(nucleolin</t> and MYLK4), cytoplasmic (MYLK4), and mitochondrial (NDUFB9) markers in the three fractions collected after the sucrose gradient ultracentrifuge. F1, fraction 1 (top); F2, fraction 2 (half); F3, fraction 3 (bottom). (B) Visualization of isolated Golgi vesicles using transmission electron microscopy (white arrows). Numerous smooth vesicles are seen, mostly formed by a single membrane, apparently empty with little dense content, mainly a light network of granular material. The presence of mitochondrial remains is also observed (black arrow). Bar represents 500 nm.
Primary Mouse Monoclonal Antibody Nucleolin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibody+nucleolin/pmc07580119-12-0-6?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
primary mouse monoclonal antibody nucleolin - by Bioz Stars, 2026-07
90/100 stars
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Analysis of Isolated Fractions (A) Western blot of Golgi (GM130 and MYLK4), nucleus (nucleolin and MYLK4), cytoplasmic (MYLK4), and mitochondrial (NDUFB9) markers in the three fractions collected after the sucrose gradient ultracentrifuge. F1, fraction 1 (top); F2, fraction 2 (half); F3, fraction 3 (bottom). (B) Visualization of isolated Golgi vesicles using transmission electron microscopy (white arrows). Numerous smooth vesicles are seen, mostly formed by a single membrane, apparently empty with little dense content, mainly a light network of granular material. The presence of mitochondrial remains is also observed (black arrow). Bar represents 500 nm.

Journal: STAR Protocols

Article Title: Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient

doi: 10.1016/j.xpro.2020.100100

Figure Lengend Snippet: Analysis of Isolated Fractions (A) Western blot of Golgi (GM130 and MYLK4), nucleus (nucleolin and MYLK4), cytoplasmic (MYLK4), and mitochondrial (NDUFB9) markers in the three fractions collected after the sucrose gradient ultracentrifuge. F1, fraction 1 (top); F2, fraction 2 (half); F3, fraction 3 (bottom). (B) Visualization of isolated Golgi vesicles using transmission electron microscopy (white arrows). Numerous smooth vesicles are seen, mostly formed by a single membrane, apparently empty with little dense content, mainly a light network of granular material. The presence of mitochondrial remains is also observed (black arrow). Bar represents 500 nm.

Article Snippet: Nucleolin primary mouse monoclonal antibody , Merk Milipore , 05-565.

Techniques: Isolation, Western Blot, Transmission Assay, Electron Microscopy, Membrane